E-viri
Recenzirano
-
Colin, Florent; Martelli, Alain; Clémancey, Martin; Latour, Jean-Marc; Gambarelli, Serge; Zeppieri, Laura; Birck, Catherine; Page, Adeline; Puccio, Hélène; Ollagnier de Choudens, Sandrine
Journal of the American Chemical Society, 01/2013, Letnik: 135, Številka: 2Journal Article
Iron–sulfur (Fe–S) cluster-containing proteins are essential components of cells. In eukaryotes, Fe–S clusters are synthesized by the mitochondrial iron–sulfur cluster (ISC) machinery and the cytosolic iron–sulfur assembly (CIA) system. In the mammalian ISC machinery, preassembly of the Fe–S cluster on the scaffold protein (ISCU) involves a cysteine desulfurase complex (NFS1/ISD11) and frataxin (FXN), the protein deficient in Friedreich’s ataxia. Here, by comparing the biochemical and spectroscopic properties of quaternary (ISCU/NFS1/ISD11/FXN) and ternary (ISCU/NFS1/ISD11) complexes, we show that FXN stabilizes the quaternary complex and controls iron entry to the complex through activation of cysteine desulfurization. Furthermore, we show for the first time that in the presence of iron and l-cysteine, an Fe4S4 cluster is formed within the quaternary complex that can be transferred to mammalian aconitase (mACO2) to generate an active enzyme. In the absence of FXN, although the ternary complex can assemble an Fe–S cluster, the cluster is inefficiently transferred to ACO2. Taken together, these data help to unravel further the Fe–S cluster assembly process and the molecular basis of Friedreich’s ataxia.
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.