This paper describes the characteristics of the seismicity in the volcanic Neapolitan area during the year 2000 recorded by the monitoring seismic network of the Osservatorio Vesuviano. In particular, a detailed analysis of the seismicity of Vesuvius is presented. We compared the seismic velocity models available for the Vesuvius area locating the earthquakes recorded in the year 2000 and on the basis of the results, we introduce for routine earthquake location the new velocity model obtained by the seismic tomography experiments (TomoVes) performed in the area. We also determined the focal mechanisms and analysed the seismicity rate, comparing the results with those obtained for the past years. After the introduction of the new acquisition system at the Osservatorio Vesuviano, a re-calibration of the duration magnitude scale was necessary to avoid biases related to the different instrumental response. Consequently, we re-calibrated the magnitude relation used for the Vesuvius earthquakes, obtaining a new formula to be used for the earthquakes recorded by the new acquisition system. Finally, we give a description of the seismic activity in the Campi Flegrei area during the summer of 2000.