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Tuschick, S; Kirischuk, S; Kirchhoff, F; Liefeldt, L; Paul, M; Verkhratsky, A; Kettenmann, H
Cell calcium (Edinburgh) 21, Številka: 6Journal Article
The endothelin (ET) isoforms ET-1, ET-2 and ET-3 applied at 100 nM triggered a transient increase in Ca2+i in Bergmann glial cells in cerebellar slices acutely isolated from 20-25 day-old mice. The intracellular calcium concentration (Ca2+i) was monitored using Fura-2-based Ca2+i microfluorimetry. The ET-triggered Ca2+i transients were mimicked by ETB receptor agonist BQ-3020 and were inhibited by ETB receptor antagonist BQ-788. ET elevated Ca2+i in Ca(2+)-free extracellular solution and the ET-triggered Ca2+i elevation was blocked by 500 nM thapsigargin indicating that the Ca2+i was released from InsP3-sensitive intracellular pools. The ET-triggered Ca2+i increase in Ca(2+)-free solution was shorter in duration. Restoration of normal extracellular Ca2+ briefly after the ET application induced a second Ca2+i increase indicating the presence of a secondary Ca2+ influx which prolongs the Ca2+ signal. Pre-application of 100 microM ATP or 10 microM noradrenaline blocked the ET response suggesting the involvement of a common Ca2+ depot. The expression of ETB receptor mRNAs in Bergmann glial cells was revealed by single-cell RT-PCR. The mRNA was also found in Purkinje neurones, but no Ca2+ signalling was triggered by ET. We conclude that Bergmann glial cells are endowed with functional ETB receptors which induce the generation of intracellular Ca2+i signals by activation of Ca2+ release from InsP3-sensitive intracellular stores followed by a secondary Ca2+ influx.
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