A proper amount of excess oxygen plays a significant role in hole‐doped cuprate high‐Tc superconductivity. Here, the dopant oxygen in Bi2Sr2CaCu2O8+δ is directly imaged via integrated differential phase contrast combined with state‐of‐the‐art scanning transmission electron microscopy. The location of dopant oxygen is observed to be consistent with the position inferred from local strain analysis of the incommensurate structure. The influence of dopant oxygen on the local atomic lattice and electronic structure is further explored using first‐principle calculations. The dopant oxygen atoms not only aggravate the distortions of the local atomic arrangement but also alter the electronic states by transferring charge from the BiO planes to the CuO2 planes. The underlying mechanism of charge transfer is resolved. The results may also be applicable to other oxygen‐doped cuprates with high‐Tc superconductivity. A proper amount of excess oxygen plays a significant role in hole‐doped cuprate high‐Tc superconductivity. The dopant oxygen in Bi2Sr2CaCu2O8+δ and the incommensurate atomic structure are directly imaged via integrated differential phase contrast combined with state‐of‐the‐art scanning transmission electron microscopy. The influence of dopant oxygen on the local atomic lattice and electronic structure is further explored using first‐principle calculations.