The aims of the present study were to investigate the effect of parity on the resumption of ovarian cyclicity and to characterize metabolic factors affecting ovarian cyclicity. The study was conducted on a total of 24 Holstein-Friesian dairy cows. The cows were divided into 2 groups comprising 12 animals each, according to parity as primiparous or multiparous. The serum samples were analysed for the indicators of metabolic status: non-esterfied fatty acids (NEFA), beta-hydroxybutyrate (BHB), glucose, triglycerides, total cholesterol, and high density lipoprotein (HDL)-cholesterol. The resumption of ovarian cyclicity was determined by progesterone concentrations and gynaecological examination. The concentrations of HDL-C and NEFA were either significantly higher or lower, respectively, in relation to the resumption of ovarian cyclicity. These findings suggested that overcoming the negative energy balance (NEB) is the most important prerequisite for determining the resumption of ovarian cyclicity. This study also revealed a significantly longer period until the first service in primiparous cows as compared to multiparous cows. In conclusion, the primiparous cows were more susceptible to metabolic stress during the transition period, and their metabolic and endocrine profiles were more unbalanced compared to the multiparous cows. Key words: negative energy balance, parity, ovarian cyclicity, dairy cows Cilj ovog rada bio je istraziti ucinak pariteta na povratak ciklicne aktivnosti jajnika i odrediti metabolicke pokazatelje koji utjecu na ciklicnu aktivnost jajnika. Istrazivanje je provedeno na ukupno 24 krave holstajnskofrizijske pasmine. Krave su bile podijeljene s obzirom na paritet u dvije skupine od po 12 zivotinja u svakoj prema paritetu na primiparne i multiparne. U uzorcima seruma analizirani su pokazatelji metabolickog statusa neesterificirane masne kiseline (engl. akronim NEFA), betahidroksi butirat (BHB), glukoza, trigliceridi, ukupni kolesterol i kolesterol iz lipoproteina visoke gustoce (engl. akronim HDL-C). Ponovno uspostavljanje ciklicne aktivnosti jajnika bilo je odredeno pomocu koncentracije progesterona i ginekoloskim pregledom. Utvrdena je znacajno visa koncentracija HDL-C i niza koncentracija NEFA u odnosu na ponovno uspostavljanje ciklicne aktivnosti jajnika. Ovi nalazi upucuju na zakljucak daje nadvladavanje NEB-a najvaznije preduvjet koji odreduje ponovno uspostavljanje ciklicne aktivnosti jajnika. Nase je istrazivanje takoder pokazalo da su primiparne krave imale znacajno duze razdoblje do prvog osjemenjivanja nakon teljenja u odnosu na multiparne krave. Stoga se moze zakljuciti da su primiparne krave osjetljivije na metabolicki stres tijekom prijelaznog razdoblja te da su njihovi metabolicki i endokrini profili neuravnotezeniji u usporedbi s onima u multiparnih krava. Kjucne rijeci: negativni energetski status, paritet, ciklicna aktivnost jajnika, mlijecna krava