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Hino, Jun; Takao, Makoto; Takeshita, Norimatsu; Konno, Yasuhiko; Nishizawa, Tsutomu; Matsuo, Hisayuki; Kangawa, Kenji
Biochemical and biophysical research communications, 06/1996, Letnik: 223, Številka: 2Journal Article
BMP-3b (also termed GDF-10) is a novel BMP-3 related protein recently discovered in rat femur tissue by molecular cloning. In this paper, we have isolated cDNA and the gene for human BMP-3b and determined their structure. Cloned human BMP-3b cDNA with a size of 2632 base pairs encodes a 478 amino acid precursor protein sharing 83% identity with rat BMP-3b. The human BMP-3b gene is composed of 3 exons and spans approximately 13 kilobases of DNA. The 5′-flanking region carries no typical TATA box but G+C rich sequences. Southern blot analysis revealed that the BMP-3b gene is situated in a single locus of chromosome 10. By Northern analysis, human BMP-3b transcripts were detected primarily in femur, brain, lung, skeletal muscle, pancreas and testis.
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in: SICRIS
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