Novel porous Co 3 O 4 nanosheets (NSs) were synthesized on the flexible carbon cloth (CC) substrate by a facile hydrothermal method and applied to construct a non-enzymatic sensor for glucose detection. The sensor is based on the electro-catalytic oxidation of glucose on the surface of Co 3 O 4 NSs. Since this particular nanostructure can provide large surface area and more active sites, the Co 3 O 4 NSs non-enzymatic sensor exhibits excellent analytical performance, such as a high sensitivity (8506 μA mM −1  cm −2 ), a fast response time (less than 6 s), low detection limit of 1 μM, good selectivity, and long-term stability. The results suggest that the porous Co 3 O 4 NSs have great potential applications in the development of sensors for enzyme-free detection of glucose.