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Kraus, J.; Horn, G.; Zimprich, A.; Simon, T.; Mayer, P.; Hollt, V.
Biochemical and biophysical research communications, 10/1995, Letnik: 215, Številka: 2Journal Article
The rat μ opioid receptor gene promoter was cloned and characterized. It has a few features in common with the mouse gene, e.g. the lack of a classical TATA box and the fact that several transcriptional start sites are used. The overall homology between the two species is greater than 85%. Functional analysis of the promoter was performed using transient expression of rat μ opioid receptor-reporter gene constructs in the μ opioid receptor expressing cell line SH SY5Y and in non μ opioid receptor expressing cell lines. A promoter region was defined which confers both high basal and TPA and forskolin stimulated reporter gene expression in SH SY5Y cells.
