Deoxynivalenol (DON) is a type B trichothecene, produced by the Fusarium species. Exposure to DON might cause disruptive effects such as reduced weight gain, neuroendocrine changes and immune modulation in animals (rats, dogs, pigs). There is huge concern that similar effects can be observed in humans. DON is a potential regulator of intracellular steroidogenesis. It is also possible that DON will be involved in the regulation of miRNAs connected with steroidogenesis. This review summarizes the latest knowledge about the influence of DON on steroidogenesis and human hormonal balance. Deoxynivalenol (DON) is a type B trichothecene, produced by the Fusarium species. DON exposure can have disruptive consequences for animals and therefore more research needs to be conducted to determine the effects of exposure for humans. This review summarizes the latest knowledge about the influence of DON on steroidogenesis and human hormonal balance.