Based on studies conducted in 2017, the features of formation and changes in the isotopic (δ 18 O and δ 2 H) and chemical composition of natural waters on the western border of the Ufa plateau were revealed. Within the Ufa plateau, waters were tested in three hydrodynamic zones of the Orda cave and the adjacent territory. Changes in the isotope-hydrochemical parameters in the horizontal and vertical directions are noted. Data from isotope-hydrochemical monitoring allowed us to establish that the replenishment of groundwater reserves on the western border of the Ufa plateau occurs mainly due to isotopically light winter precipitation, which is explained by the speed of water exchange in the karst system and the time of atmospheric precipitation in the aquifer. According to isotope data, the time of water inflow from the basin of aquifer into the aquifer was determined, which is approximately equal to six months. The latter indicates that there is some delay in the transfer of isotopic signal from the precipitation to the groundwater. The use of Spearman’s rank correlation coefficient made it possible to clarify the relationship of surface waters with waters in different hydrodynamic zones. No connection was found between the isotopic compositions of atmospheric precipitation and the waters of the vertical downward circulation zone, the connection with the composition of the waters of the horizontal circulation zone was also not established, and with the waters of the siphon circulation zone was partially established for one source. This correlation suggests that the groundwater on the western border of the Ufa plateau has a predominantly mixed aquifer recharge.