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Karnišová Potocká, Elena; Mastihubová, Mária; Čičová, Iveta; Mastihuba, Vladimír
Monatshefte für Chemie, 2018/1, Letnik: 149, Številka: 1Journal Article
Free rutinose was prepared by enzymatic hydrolysis of rutin using defatted seed meal from tartary buckwheat. This disaccharide was used as substrate in spectrophotometric assay of α- l -rhamnosidase. The assay is based on hydrolysis of rutinose and subsequent determination of released glucose by a standard glucose oxidase assay kit. The method is easy to perform and requires no expensive equipment. The assay was applied in α- l -rhamnosidase estimation in ten commercial enzyme preparations and compared with standard assay on chromogenic substrate. Graphical abstract
