E-viri
Recenzirano
-
Durante, C.; Agostini, F.; Abbruzzese, L.; T. Toffola, R.; Zanolin, S.; Suine, C.; Mazzucato, M.
Vox sanguinis, August 2013, Letnik: 105, Številka: 2Journal Article
Background and Objectives Clinical use of plasma rich in growth factors requires biochemical product control. We aimed to measure and modulate concentrations of growth factors in solutions deriving from platelet apheresis or whole blood. Materials and Methods Growth factor concentrations were measured 5’, 10’, 20’, 30’, 60’ after CaCl2 was added at 40°C to platelet‐apheresis products (n = 39) or after 60’ in platelet concentrates from whole blood (n = 13). Growth factor release was also obtained in platelet apheresis a) by incubation at 22°C or 40°C for 10’ or 30’ (n = 4); b) by repeated freeze–thaw (n = 9). Results Fibroblast growth factor (FGF), platelet‐derived growth factor (PDGF) isoforms AA and AB and transforming growth factor beta (TGF‐β) concentrations (pg/109 plt) were 25–60% higher in growth factors solutions from whole blood compared to platelet apheresis. Vascular endothelial growth factor (VEGF), TGF‐β and PDGF isoforms were released early (5–10’) during incubation: TGF‐β concentration increased also at 30’. FGF and epidermal growth factor (EGF) were released only after 30’. Incubation at 40°C/10’ increased VEGF (+70%) and decreased EGF (−30%) and PDGF‐BB (−50%) versus 22°C/30’. Shock significantly increased TGF‐β (1·6‐fold), EGF (1·5‐fold), FGF (4·5‐fold) and lowered PDGF isoforms (0·2‐ to 0·5‐fold) versus prolonged incubation at 40°C. Conclusion Platelets from platelet apheresis and whole‐blood release all investigated growth factors. The release can be regulated controlling incubation time and/or temperature and performing cell lysis.
Avtor
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.