The present study has been focused on the extraction of natural dyes from Syzygium cumini (L.) Jambolan fruit dry seed endosperm and investigation of their phytochemicals and pharmacological characteristics. Dyes were prepared using aqueous, acidic, alcoholic and alkaline extraction techniques. UV spectral studies of the dyes showed a variation in absorption maxima and their color varied with respect to the pH and the solvent used during extraction. The dye was prepared from Jambolan fruit dry seed endosperm showed good antibacterial activity. The aqueous extraction of Jambolan fruit dry seed endosperm was able to inhibit the growth of many bacterial strains viz S. lutea, E.coli, P. aeruginosa, Pseudomonas fluorescens and S. aureus etc. The antimicrobial property of the dyes was used in developing antimicrobial fabric.