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EADY, C. C; WELD, R. J; LISTER, C. E
Plant cell reports, 03/2000, Letnik: 19, Številka: 4Journal Article
An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. The method takes between 3-5 months from explant to primary regenerant entering the glasshouse. Multiple-shoot formation from primary transgenic material made possible the clonal multiplication of transformants. The binary vector used carried the nptII antibiotic resistance gene and the m-gfp5-ER reporter gene. Transgenic cultures were initially screened for their ability to fluoresce and to grow in the presence of geneticin (5-25 mg/l). The transgenic nature of individual plants was confirmed by Southern blot analysis.
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in: SICRIS
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