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KÖNIG, Julian; ZARNACK, Kathi; LUSCOMBE, Nicholas M; ULE, Jernej
Nature reviews. Genetics, 02/2012, Letnik: 13, Številka: 2Journal Article
RNA-binding proteins are key players in the regulation of gene expression. In this Progress article, we discuss state-of-the-art technologies that can be used to study individual RNA-binding proteins or large complexes such as the ribosome. We also describe how these approaches can be used to study interactions with different types of RNAs, including nascent transcripts, mRNAs, microRNAs and ribosomal RNAs, in order to investigate transcription, RNA processing and translation. Finally, we highlight current challenges in data analysis and the future steps that are needed to obtain a quantitative and high-resolution picture of protein-RNA interactions on a genome-wide scale.
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in: SICRIS
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