•The stratigraphic Mg isotopic profiles and the depositional cycles are coupled in the massive dolostone succession.•Mg isotope could be used to sequence the dolomitization events.•Massive dolostone could be generated by the temporal and spatial stacking of multiple dolomitization events.•Sea level fluctuation results in the stacking of multiple dolomitization events. The origin of ancient massive dolostones, i.e. continuous dolostone sequence with a thickness >100 m and a platform-wide distribution, is the key issue of the ‘Dolomite Problem’ that cannot be clearly demonstrated by any existing dolomitization model individually or sequentially. It has been proposed that the massive dolostone could be generated by the stacking of multistage dolomitization events linked to the sea-level fluctuation, which results in repeatedly occurring of limestone precipitation-dolomitization cycles. However, the sequence of dolomitization events cannot be differentiated by any sedimentological or traditional geochemical techniques. Here we report Mg isotopic compositions of the massive dolostone (δ26Mgdol) from the middle Cambrian Qinjiamiao Formation (QJM) in the Yangtze Platform, South China, which consists of cyclic depositions of shoaling upward sequences. The stratigraphic variation of δ26Mgdol is coincident with the depositional cycles, suggesting the dolomitization might be periodic and be coupled with the sea-level oscillation. As dolomitization fluids experience changes in δ26Mg values during dolomitization processes, the intra-cycle stratigraphic δ26Mgdol profile reflects the processes of dolomitization. Our study indicates that the massive dolostone could be generated by the temporal and spatial stacking of multiple dolomitization events that are associated with sea-level fluctuation. If this model can be verified by other massive dolostone successions, the origin of massive dolostone may be resolved.