To meet the current critical need for qualified nurses, many colleges have initiated online programs, primarily aimed towards registered nurse (RN) to BS students. Despite the growing number of online nursing programs, there is little research on instructor views of online learning. This study used interviews to investigate nursing instructor experiences in online learning. Results revealed instructor concerns with identifying the most effective assessment methods to judge students' ability to apply their lessons in real-world settings. Online nursing exhibits unique challenges, such as providing nursing students with online authentic learning experiences that relate to real-world nursing situations, which comprise both high-stake medical and interpersonal elements. Results also indicated that providing support and ensuring that the faculty have sufficient time to develop effective courses, with adequate assessment for the students, is necessary to ensure the quality of online nursing education.