Some species of bees restrict foraging to the twilight period before sunrise or after sunset. Among the plants sought by these nocturnal bees are species described as chiropterophilous, such as Caryocar brasiliense . The flowers of this species open in the evening and provide resources until dawn. We determined the pattern of flower visitation by nocturnal bees and their role in pollination and fruit set of C. brasiliense and evaluated its importance as floral resource for nocturnal bees. We analyzed the pollen composition of cell provisions of nocturnal bees of Ptiloglossa (Colletidae) and compared its scent with floral scent compounds of C. brasiliense . Moreover, we conducted a pollinator exclusion experiment to determine the contribution of nocturnal bees to its fruit set. Disregarding bats, Ptiloglossa latecalcarata and two species of Megalopta (Halictidae) were consistent nectar and pollen gathering visitors, along with some social diurnal bees. The visitor exclusion experiment revealed that bee visits do not result in fruit set, which only occurs through visits by bats. The flowers supply a significant amount of pollen for nocturnal bees, as demonstrated through pollen analysis of brood cells and scopa loads. This interaction, therefore, is only beneficial to the commensalist bees. The scent collected from brood cells was dominated by hexanoic acid and 1-hexanol and differed strongly from the floral scent of C. brasiliense . These results substantiate that bat-pollinated flowers are an important part of the food niche of nocturnal bees, which implies that they are sensorially equipped to recognize floral traits shaped by bats.