Based on the limited research available regarding bisexual individuals, there is some evidence to suggest this group may be subject to unique forms of prejudice within both heterosexual and lesbian/gay communities. To date, research in this area has relied on samples that do not reflect the diversity of the non-monosexual/plurisexual community. The present study explored the perceived experiences of anti-bisexual prejudice among a diverse group of self-identified bisexual, pansexual, queer and fluid adults (n = 235). Participants completed an online self-report survey containing 50-items concerning experiences and expressions of sexual orientation and gender identity. Results from the Anti-Bisexual Experiences Scale revealed bisexual participants reported more experiences of sexual prejudice from lesbian/gay individuals than did pansexual/queer/fluid participants. Exploratory analyses based on this finding revealed bisexual individuals also reported less subjective connection to the lesbian, gay, bisexual and transgender community than did pansexual/queer/fluid individuals. This factor, however, did not account for differences in experiences of sexual prejudice from lesbians/gay individuals. These findings challenge the grouping together of all plurisexual individuals under the label bisexual and underscore the importance of interpersonal context in the experience of sexual prejudice.