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Soni, Komal; Martínez-Lumbreras, Santiago; Sattler, Michael
Journal of molecular biology, 06/2020, Letnik: 432, Številka: 14Journal Article
The multi-domain RNA binding protein RBM5 is a molecular signature of metastasis. RBM5 regulates alternative splicing of apoptotic genes including the cell death receptor Fas and the initiator Caspase-2. The RBM5 RanBP2-type zinc finger (Zf1) is known to specifically recognize single-stranded RNAs with high affinity. Here, we study the structure and conformational dynamics of the Zf1 zinc finger of human RBM5 using NMR. We show that the presence of a non-canonical cysteine in Zf1 kinetically destabilizes the protein. Metal-exchange kinetics show that mutation of the cysteine establishes high-affinity coordination of the zinc. Our data indicate that selection of such a structurally destabilizing mutation during the course of evolution could present an opportunity for functional adaptation of the protein. Display omitted •RBM5 Zf1 shows conformational exchange conferred by a non-canonical cysteine.•Competition between two neighboring cysteines yields dynamic zinc ion coordination.•Mutation of the non-canonical cysteine residue confers structural and kinetic stability.•Ambiguous zinc coordination might be favorable for the evolution of zinc fingers.
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