E-viri
Recenzirano
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de Morais, Luis Carlos; Bernardes-Filho, Rubens; Assis, Odilio B. G
World journal of microbiology & biotechnology, 01/2009, Letnik: 25, Številka: 1Journal Article
Multilayer films were prepared through a self-assembly technique of proteases. Solutions of pepsin, lysozyme and trypsin at 10⁻⁵ M (pH 6.4, pH 6.4, and pH 7.6, respectively) were used as precursors for film building. The wettability of the film surfaces were estimated by contact angle measurements indicating a higher hydrophobicity to trypsin. This was in agreement to the calculated surface tension components. The patterns of the films were examined using atomic force microscopic images. Surfaces before and after bacteria (Escherichia coli) interactions were also characterized. The results indicate that the hydrophobicity plays a key role in bacterial adhesion and that roughness can be considered as a secondary factor.
