The molecular species of phospholipids (PLs) and glycolipids (GLs) were simultaneously characterized in the pulp and almond of the avocado fruit ( Persea americana Mill) of four varieties by means of high performance liquid chromatography–electrospray ionisation ion-trap tandem mass spectrometry. In the pulp, the predominant species of monoglycosyldiglycerides (MGD) were m/ z 796.6 (oleic/linolenic and linoleic/linoleic acids) and m/ z 800.4 (stearic/linoleic and oleic/oleic acids). One of the main diglycosyldiglycerides (DGD) both in the pulp and almond was m/ z 958.5 (oleic/linolenic); however, the pulp was also rich of m/ z 962.4 (oleic/oleic), whereas in the almond, m/ z 934.5 (palmitic/linoleic and palmitoleic/oleic) and m/ z 960.5 (oleic/linoleic and stearic/linolenic) were more abundant. In the almond, the main PL classes (phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI)) contained always palmitic/linoleic acids. α-Linolenic acid was contained as MGD (linolenic/linolenic) and DGD (linolenic/linolenic), more present in the pulp than in the almond. The major molecular species of glycocerebrosides (GCer) in the pulp and almond carried hydroxy-palmitic acid (C 16h:0)/4,8-sphyngadienine (d 18:2).