Dye-sensitized solar cells (DSSCs) that utilize natural dyes have garnered interest due to their low cost, eco-friendly manufacturing process, and competitive photovoltaic performance. However, their efficiency and stability issues have hindered their widespread implementation. To enhance their performance, this paper proposes a novel approach of modifying the photoanode with carbon dots (CDs) to align the band gap for easier carrier collection. The material properties were thoroughly characterized by examining their structural, morphological, optical, and electrical properties. In this study, titanium dioxide (TiO2) was synthesized using the microwave-assisted solvothermal method, while nitrogen-doped CDs derived from Citrus medica fruit juice were prepared using a simple hydrothermal treatment. Three sets of Natural Dye Sensitized Solar Cells (NDSSC) devices were created using co-activated photoanode (CD/TiO2) and unmodified photoanode (TiO2) with Platisol T/sp coated ITO serving as the counter electrode. Hibiscus (Hibiscus rosa-sinensis) and Onion (Allium cepa) peel extracts were utilized as sensitizers and Iodolyte HI-30 as the electrolyte. The most efficient device attained an efficiency of 3.5 % with Voc = 0.81 V and Jsc = 6.57 mA/cm2. This marks the highest efficiency reported using Hibiscus as a sensitizer with the current configuration, accompanied by prolonged device stability. This study showcases the potential of Citrus medica-derived nitrogen-doped CDs in achieving durable device stability. Display omitted •Synthesis of TiO2 using a microwave-assisted solvothermal method for DSSC photoanode application.•Modification of the photoanode with carbon dots derived from biomass.•Enhancement of photovoltaic parameters through a co-activation strategy.•Utilization of natural sensitizers for cost-effective and environmentally friendly DSSC fabrication.•Improved device stability through the use of carbon dot-modified photoanodes.