This article presents a non-complex method of producing biodegradable polylactide (PLA) fiber mats containing Al 2 O 3 -Ag nanopowder which display strong antimicrobial activities against E.coli and S. lutea . The method of preparing fiber mats was electrospinning. This article also gathers the results of the analysis of morphology and mechanical properties of both the Al 2 O 3 nanopowder and the PLA-Al 2 O 3 /Ag fiber mats. The examination of the Al 2 O 3 nanopowder was conducted with the use of a scanning electron microscope (SEM) and surface area measurements (BET). The morphology of the PLAAl 2 O 3 /Ag fiber mats was examined using SEM and TEM. The results of the study confirm the great potential of the electrospun PLA-Al 2 O 3 /Ag fiber mats for antibacterial application.