Display omitted •Saccharomyces cerevisiae AKP1 might be probiotic yeast.•AKP1 showed gastroprotective role in CRS induced ulcer in experimental rats.•AKP1 exhibited anti-inflammatory activity.•AKP1 may alleviate chances of acute cold induced gastric lesions. In this study, an isolated yeast strain AKP1 was selected (out of 10 isolates) on the basis of the cumulative probiotic score. Genotypic characterization confirmed that it was a Saccharomyces cerevisiae. Gastroprotective effect of AKP1 was evaluated by cold-induced gastric ulcer model in rats. Prior supplementation of AKP1 along with the food led to significant reduction of the cold-induced gastric lesion in stomach. The histological study clearly showed that the mucosal columnar epithelial cells of the stomach were protected from cold injury. The relative expressions of some inflammatory marker genes such as up-regulation of IL-10 and down-regulation of IL-12, IFN-γ, IFN-λ of gastric mucosa also supported the anti-inflammatory activity of AKP1. Therefore, the newly isolated yeast strain Saccharomyces cerevisiae AKP1 might be a novel candidate of probiotic with an anti-ulcerogenic potential and can be used as a therapeutic component to prevent this widespread disease.