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Gulikova, D. K.; Gainullina, E. T.; Ponsov, M. A.; Rybal’chenko, I. V.; Ryzhikov, S. B.; Fateenkov, V. N.
Journal of analytical chemistry (New York, N.Y.), 12/2013, Letnik: 68, Številka: 12Journal Article
s A rapid test for amanitins is proposed on the basis of their complexation with ethydium bromide leading to an intensively fluorescent adduct; the fluorescence band of the product has a shorter wavelength than that of ethydium bromide, which ensures the high specificity of the analysis. The fluorescence intensity in the system of ethydium bromide — amanitin grows linearly with the concentration of amanitin in the range 0.2–1.7 μM at the constant concentration of ethydium bromide, 0.6 μM. The detection limit is 1 μM, the time of the development of the analytical signal is up to 5 s.
