Objectives To assess the whitening efficiency and patient satisfaction after usage of commercially available toothpastes with the different whitening ingredients. Materials and methods This randomized controlled trial study comprised 161 participants who were randomly divided into eight groups based on used whitening or control/conventional toothpaste (Colgate Max Expert White CMEW, n = 20; Signal Daily White SDW, n = 20; Himalaya Sparkly White Herbalis HSWH, n = 20; Signal White System SWS, n = 20; Rembrandt Deeply White + Peroxide RDWP, n = 20; Splat Extreme White SEW, n = 20; Splat White Plus SWP, n = 21; and Kalodont Multi Repair control, n = 20). Tooth color and color change were evaluated using a spectrophotometer (CIELAB coordinates, ΔE ab *, ΔE 00 , whiteness index change ΔWID). Measurements were performed at baseline, 30 days and 60 days after the beginning of treatment, and 30 days after completing treatment – follow-up. Side effects and satisfaction with the whitening outcome were reported throughout the questionnaire. Results The results for ΔL*, Δa*, and Δb* showed an increase in lightness and decrease in yellowness for all tested whitening toothpastes compared to the control group after 30 days of use (p ≤ 0.05). Also, significant tooth color difference (ΔE ab *, ΔE 00 ) and whiteness index change (ΔWI D ) were recorded for all tested whitening toothpastes 30 days and 60 days from the beginning of use (p ≤ 0.05). Toothpaste containing a combination of urea peroxide and enzyme ingredients (SEW) demonstrated the highest and the longest whitening effect. Conclusions Whitening toothpastes can show perceptibly whitening effect after a short time of usage, but without a long-lasting impact. Clinical relevance Toothpaste containing a combination of enzymes and peroxides showed the best whitening performance compared to those containing only enzymes, peroxides, or abrasives. Trial registration ClinicalTrials.gov: NCT04460755