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Ivanova, B.B.; Simeonov, V.D.; Arnaudov, M.G.; Tsalev, D.L.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 05/2007, Letnik: 67, Številka: 1Journal Article
A validation of the developed new orientation method of solid samples as suspension in nematic liquid crystal (NLC), applied in linear-dichroic infrared (IR-LD) spectroscopy has been carried out using a model system dl-isoleucine ( dl-isoleu). Accuracy, precision and the influence of the liquid crystal medium on peak positions and integral absorbances of guest molecules have been presented. Optimization of experimental conditions has been performed as well. An experimental design for quantitative evaluation of the impact of four input factors: the number of scans, the rubbing-out of KBr-pellets, the amount of studied compounds included in the liquid crystal medium and the ratios of Lorentzian to Gaussian peak functions in the curve fitting procedure on the spectroscopic signal at five different frequencies, indicating important specifities of the system has been studied.
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