Three novel filamentous halophilic archaea, strains DFN5 T , RDMS1, and QDMS1, were isolated from the coastal saline soil samples of the intertidal zones located in different regions of Jiangsu Province, China. The colonies of these strains were pinkish-white due to the presence of white spores. These three strains are extremely halophilic and grew optimally at 35–37 °C and pH 7.0–7.5. Based on 16S rRNA and rpoB′ gene analysis, strains DFN5 T , RDMS1, and QDMS1 gathered together in phylogenetic trees and then clustered with the current species of the genus Halocatena showing 96.9–97.4% and 82.2–82.5% similarities, respectively. Both the 16S rRNA gene-based and rpoB' gene-based phylogenies were fully supported by the phylogenomic analysis, and the overall genome-related indexes indicated that strains DFN5 T , RDMS1, and QDMS1 should be a novel species of the genus Halocatena . Genome mining revealed that there are considerable differences in the genes related to β-carotene synthesis among these three strains and the current species of Halocatena . The major polar lipids of strains DFN5 T , RDMS1, and QDMS1 are PA, PG, PGP-Me, S-TGD-1, TGD-1, and TGD-2. The minor polar lipids, S-DGD-1, DGD-1, S 2 -DGD, and S-TeGD may be detected. According to the phenotypic characteristics, phylogenetic analysis, genomic and chemotaxonomic features, strains DFN5 T (= CGMCC 1.19401  T  = JCM 35422  T ), RDMS1 (= CGMCC 1.19411) and QDMS1 (= CGMCC 1.19410) were classified as a novel species of the genus Halocatena with the proposed name, Halocatena marina sp. nov. This is the first report of the description of a novel filamentous haloarchaeon isolated from marine intertidal zones.