Graphene-based materials present unique properties for electrochemical applications, and laser-induced conversion of polyimide to graphene is an emerging route to obtain a high-quality material for sensing. Herein we present compact and low-cost equipment constructed from an open-source 3D printer at which a 3.5-W visible (449 nm) laser was adapted to fabricate laser-induced graphene (LIG) electrodes from commercial polyimide, which resulted in electron transfer kinetic ( k 0 ) of 5.6 × 10 −3  cm s −1 and reproducibility calculated by relative standard deviation (RSD < 5%) from cyclic voltammograms of Fe(CN) 6 3−/4− using 5 different electrodes. LIG electrodes enabled the simultaneous voltammetric determination of uric acid (+ 0.1 V vs. pseudo-reference) and nitrite (+ 0.4 V vs pseudo-reference), with limit of detection (LOD) values of 0.07 and 0.27 µmol L −1 , respectively. Amperometric measurements for the detection of H 2 O 2 (applying + 0.0 V vs. Ag|AgCl|KCl (sat.) ) after Prussian blue (PB) modification and ciprofloxacin (applying + 1.2 V vs. Ag|AgCl|KCl (sat.) ) were performed under flow conditions, which confirmed the high stability of LIG and LIG-PB surfaces. The LOD values were 1.0 and 0.2 µmol L −1 for H 2 O 2 and ciprofloxacin, respectively. The RSD values (< 12%) obtained for the analysis using three different electrodes attested the precision of LIG electrodes manufactured in two designs. No sample matrix effects on the determination of ciprofloxacin in milk samples were observed  (recoveries between 84 and 96%). The equipment can be built with less than $300 and each LIG electrode costs less than $0.01. Graphical abstract