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Gómez-Meyer, Daniel; Guajardo, Nadia; Lienqueo, María Elena
Tetrahedron letters, 05/2023, Letnik: 121Journal Article
Display omitted The present work reports the evaluation of the yield, productivity and operational stability of the chemoenzymatic oxidation of 2,5-furandicarboxaldehyde (DFF) to 2,5-furan dicarboxylic acid (FDCA) catalyzed by immobilized lipase B from Candida antarctica (Novozym® 435) in a continuous packed bed microbioreactor. The highest yields and productivities were achieved using a feed for the reaction medium at 36 µL/min flow. The yield and specific productivity in continuous packed bed microbioreactor were approximately 20% and 1000 times higher than that obtained by operating in millibioreactors. The operational stability of the biocatalyst in the microbioreactor was higher than that achieved in a millibioreactor in continuous operation for 24 h. Finally, the packed bed microbioreactor is an interesting and straightforward strategy to increase the stability of the biocatalyst in continuous mode.
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