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Stoeckius, Marlon; Hafemeister, Christoph; Stephenson, William; Houck-Loomis, Brian; Chattopadhyay, Pratip K; Swerdlow, Harold; Satija, Rahul; Smibert, Peter
Nature methods, 09/2017, Letnik: 14, Številka: 9Journal Article
High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell sequencing approaches and scales readily with throughput increases.
