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Shao, Yong; Tian, Run; Duan, Jiaqi; Wang, Miao; Cao, Jing; Cao, Zhen; Li, Guangyue; Jin, Fen; Abd El-Aty, A M; She, Yongxin
Sensors, 01/2023, Letnik: 23, Številka: 2Journal Article
Glyphosate (GLYP) is a broad-spectrum, nonselective, organic phosphine postemergence herbicide registered for many food and nonfood fields. Herein, we developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads incubated with NH2-polyA and then hybridized with polyT-glyphosate aptamer and complementary DNA. Afterwards, a quantitative detection method based on qPCR was established. When the glyphosate aptamer on Mbs@dsDNA specifically recognizes glyphosate, complementary DNA is released and then enters the qPCR signal amplification process. The linear range of the method was 0.6 μmol/L−30 mmol/L and the detection limit was set at 0.6 μmol/L. The recoveries in tap water ranged from 103.4 to 104.9% and the relative standard deviations (RSDs) were <1%. The aptamer proposed in this study has good potential for recognizing glyphosate. The detection method combined with qPCR might have good application prospects in detecting and supervising other pesticide residues.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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