Entomopathogenic fungi (EPF) are the microorganisms thatspecifically infect and often kill insects and other arthropods. EPF is themost effective biocontrol agent against insects in the natural ecosystem whichcould be an effective alternative to chemical insecticides in bio-intensivepest management. Beauveria bassiana, one of the most prevalentsoil-borne entomopathogens, has virulence on insect pests. The present study isaimed to evaluate the pathogenicity of a native isolate of the entomopathogenicfungus B. bassiana isolated from the soil samples of a cotton field(Kuthukkal) in the Tirunelveli district of Tamil Nadu against Dysdercuscingulatus. Bio-efficacy trials were carried out with six differentconcentrations viz., 4.6 × 103,1.5× 104, 5.0 × 105, 2.7 × 106, 3.2 × 107,and 2.8 x 108 (spores/mL) in all the five nymphal instars and theadults of D. cingulatus. A 100% mortality was observed in higherconcentrations 2.8 x 108 (spores/mL) at 120hrs after treatment. The resultsof the present study show that the isolate seems to be highly promising in thepest management of D. cingulatus.