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Wilson, Michael R; Naccache, Samia N; Samayoa, Erik; Biagtan, Mark; Bashir, Hiba; Yu, Guixia; Salamat, Shahriar M; Somasekar, Sneha; Federman, Scot; Miller, Steve; Sokolic, Robert; Garabedian, Elizabeth; Candotti, Fabio; Buckley, Rebecca H; Reed, Kurt D; Meyer, Teresa L; Seroogy, Christine M; Galloway, Renee; Henderson, Sheryl L; Gern, James E; DeRisi, Joseph L; Chiu, Charles Y
The New England journal of medicine, 06/2014, Letnik: 370, Številka: 25Journal Article
A 14-year-old boy with severe combined immunodeficiency presented three times to a medical facility over a period of 4 months with fever and headache that progressed to hydrocephalus and status epilepticus necessitating a medically induced coma. Diagnostic workup including brain biopsy was unrevealing. Unbiased next-generation sequencing of the cerebrospinal fluid identified 475 of 3,063,784 sequence reads (0.016%) corresponding to leptospira infection. Clinical assays for leptospirosis were negative. Targeted antimicrobial agents were administered, and the patient was discharged home 32 days later with a status close to his premorbid condition. Polymerase-chain-reaction (PCR) and serologic testing at the Centers for Disease Control and Prevention (CDC) subsequently confirmed evidence of Leptospira santarosai infection.
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in: SICRIS
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