The study of migration within the Roman Empire has been a focus of the bioarchaeological and biogeochemical research during the last decade. The possible association of diet and sex, age, and funerary treatment during the 1st-4th centuries CE have been extensively explored in Britain, and Central-Southern Italy. Conversely, no knowledge is available about these processes for the North of the Italian Peninsula. In the present work we analyse a set (N = 16) of Roman inhumations from Bologna (Northern Italy, 1st-4th century CE), some of which are characterized by unusual features (prone depositions, transfixion of the skeleton by iron nails). Analysis of strontium, oxygen, nitrogen, and carbon isotopes is used to test for the possible correlation between funerary treatment, geographic origin, and diet. Here we provide the first biogeochemical data for a Northern Italian Imperial sample, wherein our results show no clear association between these variables, suggesting that funerary variability, at least in the analysed context, was shaped by a variety of heterogeneous factors, and not a representation of vertical social differences or differential geographic origins. •The association between diet, mobility, and atypical funerary treatment was tested on 16 individuals from Roman Bologna.•Individuals buried prone show higher δ15N values than the ones buried supine.•No clear association was found between funerary treatment and geographic origin.•Differential funerary treatment was motivated by various factors, and was not a reflection of varying geographic origins.