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Gadella, Jr, Theodorus W J; van Weeren, Laura; Stouthamer, Jente; Hink, Mark A; Wolters, Anouk H G; Giepmans, Ben N G; Aumonier, Sylvain; Dupuy, Jérôme; Royant, Antoine
Nature methods, 04/2023, Letnik: 20, Številka: 4Journal Article
We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems.
