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Gierlich, Johannes; Burley, Glenn A; Gramlich, Philipp M. E; Hammond, David M; Carell, Thomas
Organic letters, 08/2006, Letnik: 8, Številka: 17Journal Article
We report the development of the Cu(I)-catalyzed Huisgen cycloaddition (click) reaction for the multiple postsynthetic labeling of alkyne-modified DNA. A series of alkyne-modified oligodeoxyribonucleotides (ODNs) of increasing alkyne density were prepared, and the click reaction using various azide labels was investigated. Complete high-density conversion was observed for ODNs containing up to six consecutive alkyne functions. Compatibility of the click conditions with long DNA strands was shown using a PCR product obtained with an alkyne-modified primer.
