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Klumperman, Judith; van Rijnsoever, Carolien; Oorschot, Viola
Nature methods, 11/2008, Letnik: 5, Številka: 11Journal Article
The visualization of fluorescent proteins in living cells is a powerful approach to study intracellular dynamics. A limitation of fluorescence imaging, however, is that it lacks fine structural information; a fluorescent spot could represent an entire organelle, an organellar subdomain or even aggregates of proteins or membranes. These limitations can be overcome by immunoelectron microscopy (immunoem), which uniquely combines protein detection with ultrastructural detail.
