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Woodfine, Kathryn; Fiegler, Heike; Beare, David M.; Collins, John E.; McCann, Owen T.; Young, Bryan D.; Debernardi, Silvana; Mott, Richard; Dunham, Ian; Carter, Nigel P.
Human molecular genetics, 01/2004, Letnik: 13, Številka: 2Journal Article
We have developed a directly quantitative method utilizing genomic clone DNA microarrays to assess the replication timing of sequences during the S phase of the cell cycle. The genomic resolution of the replication timing measurements is limited only by the genomic clone size and density. We demonstrate the power of this approach by constructing a genome-wide map of replication timing in human lymphoblastoid cells using an array with clones spaced at 1 Mb intervals and a high-resolution replication timing map of 22q with an array utilizing overlapping sequencing tile path clones. We show a positive correlation, both genome-wide and at a high resolution, between replication timing and a range of genome parameters including GC content, gene density and transcriptional activity.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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