E-viri
Recenzirano
-
Shankar, Premlata; Sharp, Phillip A; Novina, Carl D; Murray, Michael F; Dykxhoorn, Derek M; Beresford, Paul J; Riess, Jonathan; Lee, Sang-Kyung; Collman, Ronald G; Lieberman, Judy
Nature medicine, 07/2002, Letnik: 8, Številka: 7Journal Article
RNA interference silences gene expression through short interfering 21 23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.
