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Keren, Leeat; Bosse, Marc; Thompson, Steve; Risom, Tyler; Vijayaragavan, Kausalia; McCaffrey, Erin; Marquez, Diana; Angoshtari, Roshan; Greenwald, Noah F; Fienberg, Harris; Wang, Jennifer; Kambham, Neeraja; Kirkwood, David; Nolan, Garry; Montine, Thomas J; Galli, Stephen J; West, Robert; Bendall, Sean C; Angelo, Michael
Science advances, 10/2019, Letnik: 5, Številka: 10Journal Article
Understanding tissue structure and function requires tools that quantify the expression of multiple proteins while preserving spatial information. Here, we describe MIBI-TOF (multiplexed ion beam imaging by time of flight), an instrument that uses bright ion sources and orthogonal time-of-flight mass spectrometry to image metal-tagged antibodies at subcellular resolution in clinical tissue sections. We demonstrate quantitative, full periodic table coverage across a five-log dynamic range, imaging 36 labeled antibodies simultaneously with histochemical stains and endogenous elements. We image fields of view up to 800 μm × 800 μm at resolutions down to 260 nm with sensitivities approaching single-molecule detection. We leverage these properties to interrogate intrapatient heterogeneity in tumor organization in triple-negative breast cancer, revealing regional variability in tumor cell phenotypes in contrast to a structured immune response. Given its versatility and sample back-compatibility, MIBI-TOF is positioned to leverage existing annotated, archival tissue cohorts to explore emerging questions in cancer, immunology, and neurobiology.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Vir: Osebne bibliografije
in: SICRIS
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