The study aimed to examine samples of different market original sheep cow and goat cheeses, in respect of the content and profile of FA with special emphasis on health-promoting FA. The content of fatty acids in the examined cheeses was highly differentiated and depended on the sort and type of cheese. The content of fatty acid groups in milk fat varied within the limits: SFA, 55.2-67.2%; SCSFA, 10.9-23.4%; BCFA, 1.6-2.9%; MUFA, 15.2-23.4%; PUFA, 1.9-4.3%; -MUFA, 1.8-6.0%; and CLA, 1.0-3.1%. From among the examined cheeses, the seasonal sheep cheeses (Oscypek) and mountain cow cheeses were characterized by the highest content of health-promoting fatty acids. The content of health-promoting fatty acids in the fat fraction of these cheeses was CLA 2.1-3.1%, -MUFA 3.5-6%, BCFA 2.7-2.9%, and SCSFA 12-18%.