The subfamily Drusinae (Trichoptera, Limnephilidae) is represented in Croatia with eight (8) species, including Ecclisopteryx asterix Malicky, 1979 recorded for the first time in Croatian fauna. The species was collected on two occasions: March 10th 2015 and March 6th 2021 in the Šumi Spring, at the Ivanščica Mt. in Hrvatsko Zagorje (North Croatia). This new finding considerably extends the distribution of this species, previously considered a microendemic of the Alps. The distribution of the two species, Drusus schmidi Botosaneanu, 1960 and D. croaticus Marinković-Gospodnetić, 1971 has been also enlarged with new findings. Within this study, nine specimens of the aforementioned species have been DNA barcoded and sequences were entered into the BOLD database. The results of this paper contribute to the ecological and faunistic knowledge of caddisflies in Croatia, with an emphasis on the subfamily Drusinae.