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Buksh, Benito F; Knutson, Steve D; Oakley, James V; Bissonnette, Noah B; Oblinsky, Daniel G; Schwoerer, Michael P; Seath, Ciaran P; Geri, Jacob B; Rodriguez-Rivera, Frances P; Parker, Dann L; Scholes, Gregory D; Ploss, Alexander; MacMillan, David W. C
Journal of the American Chemical Society, 04/2022, Letnik: 144, Številka: 14Journal Article
Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments in animal models. Here, we report μMap-Red, a proximity labeling platform that uses a red-light-excited SnIV chlorin e6 catalyst to activate a phenyl azide biotin probe. We validate μMap-Red by demonstrating photonically controlled protein labeling in vitro through several layers of tissue, and we then apply our platform in cellulo to label EGFR microenvironments and validate performance with STED microscopy and quantitative proteomics. Finally, to demonstrate labeling in a complex biological sample, we deploy μMap-Red in whole mouse blood to profile erythrocyte cell-surface proteins. This work represents a significant methodological advance toward light-based proximity labeling in complex tissue environments and animal models.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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