Abstract Spectral observations of the type-IIb supernova (SN) 2016gkg at 300–800 days are reported. The spectra show nebular characteristics, revealing emission from the progenitor star’s metal-rich core and providing clues to the kinematics and physical conditions of the explosion. The nebular spectra are dominated by emission lines of O i   λλ 6300, 6364 and Ca ii   λλ 7292, 7324. Other notable, albeit weaker, emission lines include Mg I  λ 4571, Fe ii   λ 7155, O I  λ 7774, Ca II triplet, and a broad, boxy feature at the location of H α . Unlike in other stripped-envelope SNe, the O i doublet is clearly resolved due to the presence of strong narrow components. The doublet shows an unprecedented emission line profile consisting of at least three components for each O i λ 6300, 6364 line: a broad component (width ∼2000 km s −1 ), and a pair of narrow blue and red components (width ∼300 km s −1 ) mirrored against the rest velocity. The narrow component appears also in other lines, and is conspicuous in O i . This indicates the presence of multiple distinct kinematic components of material at low and high velocities. The low-velocity components are likely to be produced by a dense, slow-moving emitting region near the center, while the broad components are emitted over a larger volume. These observations suggest an asymmetric explosion, supporting the idea of two-component ejecta that influence the resulting late-time spectra and light curves. SN 2016gkg thus presents striking evidence for significant asymmetry in a standard-energy SN explosion. The presence of material at low velocity, which is not predicted in 1D simulations, emphasizes the importance of multidimensional explosion modeling of SNe.