•Bionanocomposites were prepared as coating materials for Ras cheese.•Different ratios of TiO2 nanoparticle were used during bionanocomposite preparation.•The TiO2-NPs and bionanocomposites were characterized by XRD, SEM and TEM.•The obtained CS/PVA/TiO2 bionanocomposites displayed good WVTR, mechanical and barrier properties.•The cheese was kept free from surface mould growth up to 3 months by using the developed coating material. The aim of the present study was to prepare and characterize bionanocomposite materials, and to evaluate its use in the coating of Ras cheese. The bionanocomposite materials were made from mixture of chitosan/polyvinyl alcohol with loading of titanium dioxide nanoparticles (TiO2-NPs) from (0.5–2%). The prepared nanoparticles as well as the bionanocomposites were evaluated using, XRD, SEM, TEM, FT-IR and final contact angle. Furthermore, the mechanical properties and water vapor transmission rate (WVTR) of the fabricated bionanocomposites were evaluated. The impacts of coating Ras cheese with the prepared bionanocomposite on weight losses and microbiological, chemical, and physical characteristics of the Ras cheese were assessed during ripening in comparison to the uncoated cheese. Coating of cheese decreased the weight and moisture losses but didn't affect the normal ripening changes in the microbiological, chemical and textural properties of Ras cheese. Coating cheese with film containing 2% TiO2-NPs eliminated mold growth on the cheese surface.