Purpose of this research is to explain the factors affecting high school teachers' acceptance and use of interactive whiteboard within the scope of FATIH project according to the Unified theory of acceptance and use of technology (UTAUT, Venkatesh et al., 2003). The study was designed using causal research design in order to examine the cause-effect relationship between the variables. Sample of the study consists of 158 teachers, determined via criteria sampling, who have participated in the study voluntarily. Data has been collected using a 7 points Likert scale, developed for this study, which covers UTAUS's factors and variables featuring interactive whiteboard acceptance and use of the teachers. The collected data has been analyzed with simple and multiple linear regressions and structural equation model. The findings have shown that performance expectancy, effort expectancy and social influence had positive effects on behavioral intention; behavioral intention and facilitating conditions had positive effects on the usage time of interactive whiteboard. •Effort expectancy has a positive effect on behavioral intention regarding of interactive whiteboards.•Social influence has a positive effect on regarding the use of interactive whiteboards.•Facilitating conditions have an effect on the use of interactive whiteboards.•Facilitating conditions affect the use of interactive whiteboards in advanced ages.•Behavioral intention has an effect on the use of interactive whiteboards.