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Yoshioka, H. (Nagoya Univ. (Japan)); Yamada, N; Doke, N
Plant and cell physiology, 09/1999, Letnik: 40, Številka: 9Journal Article
Sesquiterpene cyclase and squalene synthase are key branch point enzymes in isoprenoid pathway for the synthesis of sesquiterpenoid phytoalexins and sterols/steroid glycoalkaloids, respectively. cDNA clones encoding these enzymes were isolated from potato. A phylogenetic tree showed that the sesquiterpene cyclase is vetispiradiene synthase. Infection of Phytophthora infestans with potato tubers caused transient increases in the transcript level of vetispiradiene synthase in a compatible and an incompatible interactions. On the other hand, wound expression of the squalene synthase was suppressed in favor of the expression of vetispiradiene synthase regardless of inoculated races.
