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Kim, Jihye; Ryu, Changsoo; Ha, Jongkwan; Lee, Junmyoung; Kim, Donghwi; Ji, Minkyoo; Park, Chi Soo; Lee, Jaeryong; Kim, Dae Kyong; Kim, Ha Hyung
Biomolecules, 04/2020, Letnik: 10, Številka: 4Journal Article
Bovine submaxillary mucin (BSM) is a gel-forming glycoprotein polymer, and Ser/Thr-linked glycans ( -glycans) are important in regulating BSM's viscoelasticity and polymerization. However, details of -glycosylation have not been reported. This study investigates the structural and quantitative characteristics of -glycans and identifies -glycosylation sites in BSM using liquid chromatography-tandem mass spectrometry. The -glycans (consisting of di- to octa-saccharides) and their quantities (%) relative to total -glycans (100%; 1.1 pmol per 1 μg of BSM) were identified with 14 major (>1.0%), 12 minor (0.1%-1.0%), and eight trace (<0.1%) -glycans, which were characterized based on their constituents (sialylation (14 -glycans; 81.9%, sum of relative quantities of each glycan), non-sialylation (20; 18.1%), fucosylation (20; 17.5%), and terminal-galactosylation (6; 3.6%)) and six core structure types Gal-GalNAc, Gal-(GlcNAc)GalNAc, GlcNAc-GalNAc, GlcNAc-(GlcNAc)GalNAc, and GalNAc-GalNAc. -glycosylation sites were identified using -glycopeptides (bold underlined; GE R VI, H GR R I, G P V AEQI, RP YGAL, Q LGPL, M R VVV, and RPEDN AVA) obtained from proteolytic BSM; these sites are in the four domains of BSM. The gel-forming mucins share common domain structures and glycosylation patterns; these results could provide useful information on mucin-type -glycans. This is the first study to characterize -glycans and identify -glycosylation sites in BSM.
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